Description: Crispr Guide Rna Design : Methods and Protocols, Paperback by Fulga, Tudor A. (EDT); Knapp, David J. H. F. (EDT); Ferry, Quentin R. V. (EDT), ISBN 1071606891, ISBN-13 9781071606896, Like New Used, Free shipping in the US This detailed volume focuses on the CRISPR-associated guide RNA and how it can be designed, modified, and validated for a broad repertoire of purposes. Beginning with a section on computational design of target-specific guide RNAs, th continues by covering chemical modifications to alter guide RNA stability, specificity, and efficiency, as well as to create inducible guide RNAs, append additional functional domains, and express guide RNAs in a conditional manner. It concludes with methods for measuring off-target guide RNA activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and essential, CRISPR Guide RNA Design: Methods and Protocols provides a comprehensive pipeline for guide RNA design and aims to be an invaluable resource in applying this powerful technology to basic research and therapeutic applications.
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Restocking Fee: No
Return shipping will be paid by: Buyer
All returns accepted: Returns Accepted
Item must be returned within: 14 Days
Refund will be given as: Money Back
Book Title: Crispr Guide Rna Design : Methods and Protocols
Number of Pages: X, 286 Pages
Language: English
Publisher: Springer
Publication Year: 2021
Topic: Life Sciences / Genetics & Genomics, Life Sciences / Biology, Biomedical
Illustrator: Yes
Genre: Technology & Engineering, Science
Item Weight: 20 oz
Item Length: 10 in
Author: David J. H. F. Knapp
Item Width: 7 in
Book Series: Methods in Molecular Biology Ser.
Format: Trade Paperback
